His research field covers bioenergy, environmental biotechnology, enzyme engineering and nanobiotechnology. Before joining Tsinghua University in 2001, he had ever worked in universities of Japan for ten years. His current research interests include: 1) development of Rapid and visible quantification tools for bioprocess control and optimization by using fusion protein technology; 2) real-time functional analysis and construction of a multi-species microbial coupling systems 3) construction of novel NADH regeneration systems and their applications; 4) functional analysis of new hydrogenases and their applications; 5) cell membrane engineering and control of electron transport across the cells; 6) genetic engineering of methanotrophs and the reconstructions of metabolic pathway; 7) production of soluble heparinase I fused with affinity tags and the enzymatic process for clean production of low molecular weight heparin; 8) system biological engineering of Streptomyces sps.; 9) metabolic and bioprocess engineering for enhanced biohydrogen production with higher H2 yield from biomass; 10) mechanism of atmospheric-pressure low temperature plasma acting on microorganisms and the plasma biotechnology; 11) high cell density culture of methanotrophes and their bioindustry applications; 12) biological systems for wastewater treatment capable of on-site reduction of excess sludge; 13) application of microbubble system in industrial and environmental biotechnology