In present, maintaining viability and improving biocontrol efficacy of antagonistic yeasts is a critical question which is urged to be resolved. At the same time, large scale production of antagonistic yeasts is also very important to accelerate the industrial production and commercial application. To resolve above questions, this project will mainly carry out studies from the following aspects: (1) Find cellular and physiological changes of antagonistic yeast cells during the progress of apoptosis under environmental stresses and the relationship between cell apoptosis and decrease in viability, and reduce the percentage of cell apoptosis during the producing and applying of antagonistic formula. (2) Investigate the effects of carbon source, nitrogen source, temperature, culture time, as well as other factors on the mass yield of antagonistic yeasts, and establish the producing technical systems for various yeasts, respectively; (3) Study the effects of environmental conditions on the viability and shelf-life of antagonistic yeast formula, screen the effective protectants and preserving technology to improve the viability.

From the study, we can find the cellular and physiological characters and regulating mechanisms during the progress of apoptosis, determine the main factors which affect the viability and biocontrol efficiency of antagonistic yeasts, and find the optimum culture conditions, such as carbon source, nitrogen source, temperature and culture time. Then, the technical systems of production and storage will be integrated. After this study, we can establish the technical base for the industrial production and commercial application of antagonistic yeasts, and take the relative field in China into the leading level in the world around.