Biocatalysis is playing more and more important roles in the developments of bio-based products and bio-energies. In this project we propose to construct multienzymatic systems with high efficiency through a multi-level designof biocatalysts, i.e., biomolecular machinery. With this biomolecular machinery, the synthesis of important chemical and material based on natural fat will be studies. To construct the biomolecular machinery, the key scientific problem has to be solved is how to achieve an effective coordination among nanostructure material supported enzymes and coenzymes by using and promoting the unique properties of nanostructure material. The targeting reaction system is the simultaneous synthesis of 1, 3-dihydroxyacetone and xylitol from glycerol and xylose by coupling the reactions of two enzymes, glycerol dehydrogenase and xylose reductase. With this coupling reaction, a in-situ regeneration and recycling of coenzyme NAD(H) will be achieved.
